Arrey 1, xiaoyue jiang 2, eugen damoc, rosa viner 2, yue xuan 1, zeller martin, michaela scigelova, thomas moehring, markus kellmann 1. Thermo scientific q exactive benchtop quadrupoleorbitrap mass spectrometer identify, quantify and confirm with unmatched confidence using the q exactive mass spectrometer. Thermo scientific q exactive hfx hybrid quadrupoleorbitrap ms system product specifications the thermo scientifi c q exactive hfx hybrid quadrupoleorbitrap mass spectrometer combines a highresolution, accuratemass hram ultrahighfi eld. Exactive series operating manual revision a thermo fisher. This will inform you about anything unusual that occurred before, and solvents were used before. Notice never change the settings in this window except during installation of the instrument.
Q exactive aka amelie this instrument was sent by thermo scientific to start our fleet of exactive series instruments. Demonstrates how to remove and clean the slens on the q exactive instrument series removing and cleaning the slens on thermo scientific q exactive mass spectrometers on vimeo join. Demonstrates how to remove the ionsource interface on the q exactive instrument series. Q exactive hf hybrid quadrupoleorbitrap mass spectrometer. Thermo q exactivewelcome to our storenewsletter send message homeabout ussell your equipmentour feedbackadd to favoritescontact us fast shippingsatisfaction gu. Thermaqs large backlit display delivers the right temperature info without having to push confusing buttons and with none of the useless fluff found in houseware products. Q exactive series of mass spectrometers consist of rflens, bent. The thermo scientific q exactive hf hybrid quadrupoleorbitrap mass spectrometer features an ultrahighfield orbitrap analyzer which doubles its speed and resolution more samples run and amazing data quality. Thermo scientific q exactive hf orbitrap lcmsms system higherquality data, faster than ever the thermo scientific q exactive hf benchtop lcmsms combines a highresolution accuratemass hram ultrahighfield orbitrap analyzer and a high performance quadrupole precursor ion selection to deliver unsurpassed speed while. This guide is intended for all personnel who operate the q exactive plus mass spectrometer.
Check log sheet to see the notes of previous users. Isobaric mass tagging quantification using q exactive. Esi and apci source uhplc system ultimate 3000 dionex nanomate fraction collectorinjection robot advion available pdadetector thermo. Q exactive hf software manual thermo fisher scientific. Samples were analyzed on a thermo q exactive orbitrapequipped with xrstm uhplc pump and open accelaautosampler. Thermo scientific q exactive mass spectrometer series calibration and maintenance. The thermo scientific q exactive plus benchtop lcmsms combines high performance quadrupole precursor selection with highresolution, accurate mass. What you need to know about calibration and maintenance of the thermo scientific q exactive mass spectrometer series. Built on the proven performance of the q exactive plus platform, the q exactive hf mass spectrometer. Identify and quantify more proteins, peptides, lipids, glycans and small molecules accurately and in less time with the thermo scientific q exactive hf hybrid quadrupoleorbitrap mass spectrometer. A next generation system for quantitation the thermo. This guide is intended for all personnel who operate the q exactive mass spectrometer.
Refurbished thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer. The thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer introduced the revolutionary concept of quanfirmation. The superior quality of q exactive msms data provides ultimate confidence for a wide range of qualitative and quantitative applications. The q exactive plus hybrid quadrupoleorbitrap mass spectrometer takes quanfirmation to the next level with increased performance and new options that enhance applications from confident dmpk qual.
You can access pdf files of the documents listed above from the data system computer. Refer to the q exactive operating manual for instructions about preparing the positive ion mode calibration solution or buying the readytouse solution from thermo fisher scientific. It also proposes methods to test prm and tsim workflows with thermo. The thermo scientific q exactive gc orbitrap gcmsms system and the new thermo scientific exactive gc orbitrap gcms system have changed all of that. If you have a q exactive gc system, go to start all programs thermo instruments manuals. Warning symbols alert the user to check for hazardous conditions. Pdf introduction 120 words thermo scientific has recently introduced two new mass spectrometers ms. The thermo q exactive plus, paired with the biopharma finder 3. With a fast scan speed and multiplexing capabilities, the q exactive mass spectrometer is an outstanding detector for. The superior quality of q exactive plus msms data enables identification and quantitation of more compounds with greater confidence. Need help with missing mz peak in qexactive calibration. Removing and cleaning the slens on thermo scientific q. Lcms quantitative screening method for 18 anabolic.
The q exactive hf system combines a stateoftheart segmented quadrupole for highperformance precurs. Quantification and qualification of oligonucleotides by high resolutionaccurate mass orbitrap ms hongxia jessica. Thermo q exactive the exactive plus orbitrap lcms is a benchtop full scan mass spectrometer designed for compound identification and highthroughput, highperformance screening. The thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection. Resolving power up to 140,000 maximum scan speed 12 hz intrascan dynamic range 5000. Thermo q exactive hf from 2017, biopharm, in excellent. Thermo scientific q exactive august 28, 20 zurichschlieren ch biognosys ag, a swiss proteomics company, announced today the second release of its spectronaut software for analysis of hyper reaction monitoring hrm data. Any changes in this window affect the data acquisition or the functionality of your instrument. Q exactive hf hybrid quadrupoleorbitrap mass spectrometers adds superior. I have an exactive plus thermo orbitrap and the ultra high vacuum turbo pump just failed. To develop a sensitive method for quantitative screening of 18 anabolic steroids in oral fluid. Thermo fisher scientific the thermo q exactive mass spectrometer has recently come off lease and is ready for your lab. Q exactive benchtop quadrupoleorbitrap mass spectrometer.
Thermo q exactive hf from 2017, biopharm, in excellent condition. Q exactive online topdown analysis generates extensive sequence information for reduced mab, offering a fast way to confirm sequence identity. Rapid peptide mapping via automated integration of online digestion, separation and mass spectrometry for the analysis of therapeutic proteins esther lewis1, kevin meyer2, zhiqi hao1, nick herold2 and patrick bennett1 1thermo fisher scientific, san jose, california, 2perfinity biosciences, inc. This q exactive plus quickstart guide gives an introduction on setting up and using the thermo scientific q exactive plus mass spectrometer. This q exactive quickstart guide gives an introduction on setting up and using the thermo scientific q exactive mass spectrometer. Stability evaluation of sigmamab msqc8 antibody drug. We also continued to expand our flagship orbitrap mass spectrometry franchise, introducing the idx tribrid and q exactive uhmr systems for applications ranging from. Pdf the q exactive hf, a benchtop mass spectrometer with a pre. Isobaric mass tagging quantification using q exactive instruments approach and expectations tabiwang n. Identify, quantify and confirm more compounds rapidly and with confidence using the thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer.
Lcms application leader life sciences mass spectrometry. Q exactive hybrid quadrupoleorbitrap mass spectrometer. Q exactive high resolution msms analysis keckes, s. Does anyone have an opinion on whether the thermo q. Calibrating thermo exactive with the direct analysis in. Ive never had a turbo fail in my years i know, im lucky.
Product specifications thermo scientific q exactive. Orbitrap mass spectrometer technology has revolutionized the. A next generation system for quantitation the thermo scientific q exactive kevin j. Thermo q exactive hfq exactive hf system features a new ultrahighfield orbitrap mass analyzer that, while physically smaller, provides higher resolution and. The q exactive benchtop lcmsms combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection to deliver high performance and tremendous versatility. Exactive series operating manual pn bre0012255, revision a. Quantification and qualification of oligonucleotides by. Thermo scientific q exactive hf biopharm from 2017 condition. Thermo exactive orbitrap mass spectrometer direct analysis in real time dart svp ion source chemyx fusion 100 syringe pump or equivalent. With a fast scan speed and multiplexing capabilities, the q exactive plus mass spectrometer is an outstanding detector for fast chromatography separation techniques. Rapid level 3 characterization of biologics using a cesi 8000 thermo q exactive platform comprehensive qualitative and quantitative analysis of biopharmaceuticals using cesims technology bryan fonslow and andras guttman sciex separations, brea, ca in the biopharmaceutical industry, comprehensive and. Rapid peptide mapping via automated integration of online.
Q exactive plus orbitrap lcmsms system thermo fisher scientific. Thermo fisher scientific exactive operating manual vii identifying safety information the exactive operating manual contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. Thermo scientific exactive series operating manual pn bre0012255, revision a vii technical data for exactive series systems the table summarizes the most import ant technical data of the exactive series systems. Among the highlights, we launched a new line of our vanquish duo liquid chromatography systems to help scientists in biopharma labs be more productive. Biopharma option for the thermo scientific q exactive plus and. This benchtop lcmsms system combines quadruple precursor ion selection with highresolution, accuratemass. Write down your name, date, startend time, sample type, and ion source information on the log sheet. Q exactive hf is a member of the thermo scientific family of mass spectrometer ms detectors that are powered by orbitrap technology. Isq lt, tsq 8000 evo, and q exactive gc mass spectrometers. It presents suggestions based on sample complexity and number of peptides to quantify.
See the respective chapters of the manual for details and additional instrument properties. Please note that this guide does not contain any safe ty notices and precautionary statements. Removing the ion source interface on thermo scientific q. Tsq 8000 evo, and q exactive gc direct probe system user guide, pn 1r1205050006 to view product manuals if you have an isq lt or a tsq 8000 evo system, open the manuals folder on your desktop. I am currently working with a q exactive focus from thermo and within 3 month, 2 electronic. Used thermo fisher q exactive certified genetool, inc. Pdf the quadrupole orbitrap mass spectrometer q exactive has made a powerful proteomics. This early stage instrument does not run with the newly designed quadrupole and bent flatupole, but one long octopole and a bent quadrupole. Q exactive ms produces accurate and reproducible mass analysis for intact mab analysis. The thermo scientific q exactive benchtop lcmsms is the next step in that revolution, combining highperformance quadrupole precursor. Exactive, orbitrap, orbitrap fusion, thermo scientific, ultimate, unity, and xcalibur are. Exactive operating manual thermo fisher scientific.
Thermo scientific q exactive hf software manual pn 72090, revision a 11. Thermo scientific q exactive hf software manual pn 72090, revision a i read this first welcome to the thermo scientific q exactive hf system. Rapid level 3 characterization of biologics using a cesi. Please contact us for complete refurbishedused thermo q exactive mass spectrometer system, warranty and service contract details. Print pdf find methods for your needs pesticide residues screening analysis in tea and honey using a q exactive focus highresolution mass spectrometer be the first to rate this application. Protein deconvolution suite enables fast and accurate calculation of the intact mass of mabs.
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